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Pré-publication, Document de travail

Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients

Abstract : Background. The systemic antibody responses to SARS-CoV-2 in COVID-19 patients has been extensively studied. However, much less is known about the mucosal responses in the upper airways at the site of initial SARS-CoV-2 replication. Local antibody responses in the nasopharyngeal epithelium, that are likely to determine the course of infection, have not been analysed so far nor their correlation with antibody responses in serum. Methods. The IgG and IgA antibody responses were analysed in the plasma as well as in nasopharyngeal swabs (NPS) from the first four COVID-19 patients confirmed by RT-qPCR in France. Two were pauci-symptomatic while two developed severe disease. Taking advantage of a comprehensive series of plasma and nasopharyngeal samples, we characterized their antibody profiles from the second week post symptoms onset, by using an in-house ELISA to detect anti-SARS-CoV-2 Nucleoprotein (N) IgG and IgA. Results. Anti-N IgG and IgA antibodies were detected in the NPS of severe patients. Overall, the levels of IgA and IgG antibodies in plasma and NPS appeared specific to each patient. Conclusions. Anti-N IgG and IgA antibodies are detected in NPS, and their levels are related to antibody levels in plasma. The two patients with severe disease exhibited different antibody profiles that may reflect different disease outcome. For the pauci-symptomatic patients, one showed a low anti-N IgG and IgA response in the plasma only, while the other one did not exhibit overt serological response.
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Pré-publication, Document de travail
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03147086
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Soumis le : vendredi 19 février 2021 - 15:34:51
Dernière modification le : mardi 21 juin 2022 - 03:42:11
Archivage à long terme le : : jeudi 20 mai 2021 - 19:44:33

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Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale - Pas de modification 4.0 International License

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Bernadette Crescenzo-Chaigne, Sylvie Behillil, Vincent Enouf, Nicolas Escriou, Stéphane Pêtres, et al.. Nasopharyngeal and serological anti SARS-CoV-2 IgG/IgA responses in COVID-19 patients. 2021. ⟨pasteur-03147086v1⟩

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